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Lab-on-a-disk extraction of PBMC and metered plasma from whole blood: An advanced event-triggered valving strategy

Uddin, Rokon, Kinahan, David J. orcid logoORCID: 0000-0003-1968-2016, Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897 and Boisen, Anja orcid logoORCID: 0000-0002-9918-6567 (2021) Lab-on-a-disk extraction of PBMC and metered plasma from whole blood: An advanced event-triggered valving strategy. Biomicrofluidics, 15 (6). ISSN 1932-1058

In this paper, we present a centrifugal microfluidic concept employing event-triggered valving for automated extraction of metered plasma and peripheral blood mononuclear cells (PBMCs). This “lab-on-a-disk” system has been developed for retrieving different density layers from a liquid column by “overflowing” the layers sequentially using the pressure exerted by a density-gradient liquid. Defined volumes of plasma and PBMCs were efficiently forwarded into designated microfluidic chambers as a sample preparation step prior to further downstream processing. Furthermore, the extracted PBMCs were counted directly on-disk using an automated optical unit by object-based image analysis, thus eliminating the requirement for the post-processing of the extracted PBMCs. This study is a direct continuation of our previous work1 where we demonstrated combined on-disk detection of C-reactive protein and quantification of PBMCs following on-disk extraction of plasma and PBMCs from a single blood sample using a centrifugo-pneumatic valving mechanism. However, the former valving technique featured limited PBMC extraction efficiency. Here, integrating the novel concept along with event-triggered valving mechanism, we eliminated the occurrence of a specific microfluidic effect, which led us to increase PBMC extraction efficiency to 88%. This extraction method has the potential to be utilized for efficiently separating multiple density layers from a liquid sample in relevant biomedical applications.
Item Type:Article (Published)
Subjects:Engineering > Biomedical engineering
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Engineering and Computing > School of Mechanical and Manufacturing Engineering
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Publisher:American Institute of Physics (AIP)
Official URL:https://dx.doi.org/10.1063/5.0066128
Copyright Information:© 2021 Authors
Funders:European Research Council under the EU’s Seventh Framework Programme (No. FP7/2007-2013)/ERC under (Grant Agreement No. 320535), Center of Excellence “IDUN” granted by the Danish National Research Foundation (Grant No. DNRF122), Velux Foundations
ID Code:27741
Deposited On:14 Sep 2022 09:33 by David Kinahan . Last Modified 26 Sep 2022 16:16

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