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Novel blood platelet diagnostic platform

Panzera, Aurora (2011) Novel blood platelet diagnostic platform. Master of Arts thesis, Dublin City University.

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Abstract

This project involves the design and implementation of a platform to investigate the activation state of platelets. The nal aim is to develop an assay to be used for diagnostic purposes. Blood platelets play a key role in the cardiovascular system. When the en- dothelium of the vessel wall is damaged platelets adhere and become activated, leading to platelet aggregation to maintain physiological haemostasis. Any abnormality in platelet functionality induces thrombus or atherosclerotic plaques leading to life-threatening consequences such as stroke. Recent reports suggest that the detection of activated platelets in the blood may be a useful indicator of the on-set of stroke and other cardiovascular abnormalities. Hence, the detection of the functional state of platelets is of huge clinical importance. A luminescence-based platelet bio-assay which uses high brightness silica nanoparticles (NPs) as super-bright labels is an innovative and promising tool for diagnostics. Compared to assays carried out using conventional single molecule dye labels, the usage of NPs makes it possible to enhance the detection eciency of the assay and to achieve a reduced limit-of-detection. In this work NPs doped with a near infra-red dye are synthesized and their interactions with the platelets are characterized. Protocols of conjugation of antibodies towards platelet surface antigens to NPs are tested. The second part of the work studies the activation state and the morphology of platelets in dierent environments by using single molecule dye label assays and with AFM. The connement of platelets in micrometer sized area using a protein patterning technique makes the analysis innovative and potentially very useful.

Item Type:Thesis (Master of Arts)
Date of Award:November 2011
Refereed:No
Supervisor(s):McDonagh, Colette
Uncontrolled Keywords:thrombus; atherosclerotic plaques; platelets; high brightness silica nanoparticles; NPs
Subjects:Physical Sciences > Nanotechnology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. View License
ID Code:16634
Deposited On:02 Dec 2011 10:39 by Colette McDonagh. Last Modified 02 Dec 2011 10:39

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