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Investigations into the sensing capabilities of an electrochemical immunosensor employing polyaniline as a mediator species

Walsh, Padraic (2006) Investigations into the sensing capabilities of an electrochemical immunosensor employing polyaniline as a mediator species. Master of Science thesis, Dublin City University.

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Abstract

The aim of this thesis was to expand the sensing capabilities of a conducting polymer-based sensor platform developed in our laboratory. The platform itself is composed of a polyaniline - poly(vinylsulphonate) (PANIIPVS) modified screen-printed carbon paste electrode used as a substrate for the immobilisation of antibody, and the subsequent competition assays of protein analytes. Chapter 1 is a literature review encompassing the field of electrochemical immunosensing. chapter 3 outlines work towards developing real-time imrnunosensors to oestradiol and p60. This ork involved the synthesis of horseradish peroxidase (HRP) conjugates with oestradiol, and p60 or use in a competitive immunosensor, as well as immunoassay characterisation of the proteins, nd their application to the biosensor platform. Chapter 4 outlines efforts to address sub-monolayer protein immobilisation issues to the PANIIPVS matrix by optimising experimental parameters overning the electrostatic irnmobilisation process. It also details experiments probing icroperoxidase-11 as an alternative labeling enzyme to HRP. Chapter 5 deals with the investigation of a novel surfactant-based strategy to reduce non-specific interactions between species present in the bulk solution, and the PANIIPVS matrix. This research highlights the need for developing the technology for real-time electrochemical immunosensing, and examines some of the critical issues involved.

Item Type:Thesis (Master of Science)
Date of Award:2006
Refereed:No
Supervisor(s):Smyth, Malcolm R. and Killard, Anthony J. and Morrin, Aoife
Uncontrolled Keywords:sensors; polyaniline; PANI; electrochemical immunosensing; PANI/PVS matrix
Subjects:Physical Sciences > Chemical detectors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. View License
ID Code:17000
Deposited On:14 May 2012 16:04 by Fran Callaghan. Last Modified 14 May 2012 16:04

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