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Stability properties of an ancient plant peroxidase

Loughran, Noeleen B. and O'Connell, Mary J. and O'Connor, Brendan and Ó Fágáin, Ciarán (2014) Stability properties of an ancient plant peroxidase. Biochimie, 104 . pp. 156-159. ISSN 0300-9084

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Abstract

Plant (Class III) peroxidases have numerous applications throughout biotechnology but their thermal and oxidative stabilities may limit their usefulness. Horseradish peroxidase isoenzyme C (HRPC) has good catalytic turnover and is moderately resistant to heat and to excess (oxidizing) concentrations of hydrogen peroxide. In contrast, HRP isoenzyme A2 (HRPA2) has better oxidative but poorer thermal stability, while soybean peroxidase (SBP) displays enhanced thermal stability. Intrigued by these variations amongst closely related enzymes, we previously used maximum likelihood methods (with application of Bayesian statistics) to infer an amino acid sequence consistent with their most recent common ancestor, the ‘Grandparent’ (GP). Here, we report the cloning and expression of active recombinant GP protein in Escherichia coli. GP’s half-inactivation temperature was 45 oC, notably less than HRPC’s, but its resistance to excess H2O2 was 2-fold greater. This resurrected GP protein enables a greater understanding of plant peroxidase evolution and serves as a test-bed to explore their ancestral properties.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Ancestral protein; Plant peroxidase; Thermal stability; Oxidative stability
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Bioinformatics
Biological Sciences > Biochemistry
Biological Sciences > Enzymology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.biochi.2014.05.012
Copyright Information:© 2014 Elsevier
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:20502
Deposited On:25 Mar 2015 11:15 by Ciaran Fagan. Last Modified 13 Feb 2017 11:51

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