Development of an autonomous algal toxin analytical platform for aquatic monitoring
Maguire, Ivan and Fitzgerald, Jenny and Heery, Brendan and Murphy, Caroline and Nwankire, Charles and O'Kennedy, Richard and Ducrée, Jens and Regan, Fiona (2016) Development of an autonomous algal toxin analytical platform for aquatic monitoring. In: Europtrode 2016, 20-23 Mar 2016, Graz, Austria.
This is the latest version of this item.
Full text available as:
Cyclic peptide cyanobacterial toxins, in particular Microcystis aeruginosa, pose a serious health risk to humans and animals alike , . Occurring mostly in fresh and brackish water, they have been identified to cause cancer promotion and liver damage . Herein, we describe a portable, microfluidic-based system for in-situ detection of algal toxins in fresh water.
The technology development presented here is a fully integrated and portable sample-to-answer centrifugal microfluidics-based system for the detection of toxic cyanobacteria – Microcystin-LR in fresh water. Our unique system employs highly-specific recombinant chicken anti-microcystin antibodies, prepared in-house, with a 3D-printed ‘LASER-photo¬diode’ fluorescent detection technique, also developed in-house. The system has high analytical specificity and sensitivity for detection of toxins below the regulatory limit with intra/inter day coefficient of variation of less than 20%. Dissolvable-film based valving technique was used for flow actuation and integration of multiple assays on the centrifugal cartridge. This new approach forms the basis of a cost efficient, USB-controlled water quality monitoring system.
Technically, this integrated system consists of two components; a microfluidic disc (figure 1.A), the disc-holder fabricated and assembled from a 3D-printed casing, with electronic components housed in device. The 5-layered microfluidic disc consists of five reservoirs (figure 1.B), each with a separate venti-lation, aligned radially with inter-connected microchannels. A competitive immunoassay format is utilised to detect free toxin (figure 1.C). Sensitivity, reproducibility and ease-of-use are key features of this monitoring device. The ‘top-down’ optical detection system has been modified for improved detection sensitivity, as well as the elimination of external noise.
Available Versions of this Item
- Development of an autonomous algal toxin analytical platform for aquatic monitoring. (deposited 27 Jun 2016 11:07) [Currently Displayed]
Archive Staff Only: edit this record