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Development of an autonomous algal toxin analytical platform for aquatic monitoring

Maguire, Ivan and Fitzgerald, Jenny and Heery, Brendan and Murphy, Caroline and Nwankire, Charles and O'Kennedy, Richard and Ducrée, Jens and Regan, Fiona (2015) Development of an autonomous algal toxin analytical platform for aquatic monitoring. In: Europtrode 2016, 20-23 Mar 2016, Graz, Austria.

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Abstract

Cyclic peptide cyanobacterial toxins, in particular Microcystis aeruginosa, pose a serious health risk to humans and animals alike [1], [2]. Occurring mostly in fresh and brackish water, they have been identified to cause cancer promotion and liver damage [3]. Herein, we describe a portable, microfluidic-based system for in-situ detection of algal toxins in fresh water. The technology development presented here is a fully integrated and portable sample-to-answer centrifugal microfluidics-based system for the detection of toxic cyanobacteria – Microcystin-LR in fresh water. Our unique system employs highly-specific recombinant chicken anti-microcystin antibodies, prepared in-house, with a 3D-printed ‘LASER-photo¬diode’ fluorescent detection technique, also developed in-house. The system has high analytical specificity and sensitivity for detection of toxins below the regulatory limit with intra/inter day coefficient of variation of less than 20%. Dissolvable-film based valving technique was used for flow actuation and integration of multiple assays on the centrifugal cartridge. This new approach forms the basis of a cost efficient, USB-controlled water quality monitoring system. Technically, this integrated system consists of two components; a microfluidic disc (figure 1.A), the disc-holder fabricated and assembled from a 3D-printed casing, with electronic components housed in device. The 5-layered microfluidic disc consists of five reservoirs (figure 1.B), each with a separate venti-lation, aligned radially with inter-connected microchannels. A competitive immunoassay format is utilised to detect free toxin (figure 1.C). Sensitivity, reproducibility and ease-of-use are key features of this monitoring device. The ‘top-down’ optical detection system has been modified for improved detection sensitivity, as well as the elimination of external noise.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:No
Uncontrolled Keywords:Aquatic monitoring; Microcystin toxins
Subjects:Physical Sciences > Analytical chemistry
Physical Sciences > Chemical detectors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Marine and Environmental Sensing Technology Hub (MESTECH)
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Copyright Information:© 2016 The Authors
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:21912
Deposited On:14 Aug 2017 16:26 by Ivan Maguire. Last Modified 14 Aug 2017 16:26

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