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Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotube-modified electrodes

Luo, Xiliang and Killard, Anthony J. and Smyth, Malcolm R. (2006) Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotube-modified electrodes. Electroanalysis, 18 (11). pp. 1131-1134. ISSN 1040-0397

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Abstract

The direct electrochemistry of glucose oxidase (GOD) was revealed at a carbon nanotube (CNT)-modified glassy carbon electrode, where the enzyme was immobilized with a chitosan film containing gold nanoparticles. The immobilized GOD displays a pair of redox peaks in pH 7.4 phosphate buffer solutions (PBS) with the formal potential of about -455 mV (vs. Ag/AgCl) and shows a surface-controlled electrode process. Bioactivity remains good, along with effective catalysis of the reduction of oxygen. In the presence of dissolved oxygen, the reduction peak current decreased gradually with the addition of glucose, which could be used for reagentless detection of glucose with a linear range from 0.04 to 1.0 mM. The proposed glucose biosensor exhibited high sensitivity, good stability and reproducibility, and was also insensitive to common interferences such as ascorbic and uric acid. The excellent performance of the reagentless biosensor is attributed to the effective enhancement of electron transfer between enzyme and electrode surface by CNTs, and the biocompatible environment that the chitosan film containing gold nanoparticles provides for immobilized GOD.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:glucose oxidase; carbon nanotubes; biosensor; direct electrochemistry; reagentless;
Subjects:Physical Sciences > Chemical detectors
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:John Wiley & Sons
Official URL:http://dx.doi.org/10.1002/elan.200603513
ID Code:44
Deposited On:08 Nov 2006 by DORAS Administrator. Last Modified 30 Jan 2009 11:08

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