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Visible spectroelectrochemical characterization of geobacter sulfurreducens biofilms on optically transparent indium tin oxide electrode

Jain, Anand, Gazzola, Giulio, Panzera, Aurora, Zanoni, Michele and Marsili, Enrico (2011) Visible spectroelectrochemical characterization of geobacter sulfurreducens biofilms on optically transparent indium tin oxide electrode. Electrochimica Acta, 56 (28). pp. 10776-10785. ISSN 0013-4686

We report visible spectroelectrochemical (SEC) characterization of cytochrome c552 (cyt c552) in viable Geobacter sulfurreducens biofilms on tin-doped indium oxide (ITO) electrodes poised at 0.24 V vs.. SHE. G. sulfurreducens biofilms were grown in minimal medium with acetate as electron donor (turnover conditions), followed by 24 hours incubation in electron donor-depleted medium (non-turnover conditions). The electronic absorption spectra of G. sulfurreducens biofilms showed the lowest energy absorption band in the reduced state at 552 nm, which indicated excess of cyt c552 in the biofilm. The spectra under non-turnover conditions displayed gradual reduction of the cyt c552, following the step-wise decrease of electrode potential from 0.0 V to -0.6 V vs.. standard calomel electrode (SCE). The spectral changes were fully reversible in both positive and negative direction of the scan potential, with average midpoint potential value of -0.42 V vs.. SCE. Confocal microscopy analysis revealed that the thickness of biofilms under turnover conditions and non-turnover conditions was approximately 35 and 3.5 µm, respectively. This is the first study to observe the reversible redox conversion of cyt c552 in viable G. sulfurreducens biofilms.
Item Type:Article (Published)
Uncontrolled Keywords:Electroactive biofilms; Geobacter sulfurreducens; Visible spectroscopy; Cytochrome c; Cyclic voltammetry
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Microbiology
Physical Sciences > Electrochemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Institutes and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier Ltd.
Official URL:https://doi.org/10.1016/j.electacta.2011.02.073
Copyright Information:© Elsevier Ltd. 2011
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Irish Research Council for Science, Engineering and Technology (IRCSET) – EMPOWER fellowship, EU-FP7 Marie Curie International Reintegration Program
ID Code:16230
Deposited On:06 Dec 2011 09:32 by Enrico Marsili . Last Modified 01 Mar 2022 13:42

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