Introduction: Postprandial lipidemia (PPL), defined as an increase in plasma levels of triglyceride-rich lipoproteins following the consumption of a high fat meal (HFM) is associated with endothelial dysfunction. Acute exercise reduces PPL and maintains endothelial function (EF) in healthy adults. The effect of acute exercise on PPL and endothelial function has not been studied in patients with peripheral arterial disease (PAD). Purpose: To examine the effect of an acute bout of exercise on PPL, vascular inflammation and endothelial function in men with PAD. Methods: Men (n=8) with PAD underwent two oral fat tolerance tests (OFTT). On the evening prior to each OFTT, participants rested (control), or exercised until they expended 200 Kcal. Blood samples were obtained at baseline and 30 min, 1, 2, 3 and 4 h postprandial. Endothelial-dependent dilation (EDD) and endothelial-independent dilation (EID) were measured in the brachial artery using ultrasonography at baseline, 2 h and 4 h postprandial. Results: Postprandial TG increased significantly and EDD decreased significantly following the OFTT. An acute bout of discontinuous exercise that resulted in a 200 Kcal expenditure did not significantly attenuate the post prandial TG response or significantly ameliorate the decrease in endothelial vasomotor function. Compared to baseline values, circulating leukocytes, and TNF-α increased (p<0.05) in both conditions 4 h postprandial. There were no changes in C-Reactive Protein (CRP). Conclusion: Prior exercise has no effect on PPL or EDD following an OFTT in men with PAD.