The ability of lectins to recognise and bind specific oligosaccharide structures has been exploited frequently in analytical platforms. However, a major difficulty encountered in lectin-based analysis, particularly in multi- well plate formats, such as Enzyme-Linked Lectin Assays (ELLA’s), is a lack of appropriate blocking solutions to prevent non-specific interactions during the course of an experiment. We have attempted to address this issue by assessing a range of blocking reagents using a panel of 19 biotinylated lectins exhibiting diverse structures and carbohydrate specificities.