The fucose binding LecB protein is one of two identified lectins produced by the opportunistic pathogen Pseudomonas aeruginosa (PA01) and is implicated in contributing to its virulence. A large number of homologous proteins have been identified in other bacterial species that exhibit extremely high sequence identity and similarity to LecB. However, key amino acid residues known to participate in fucose binding in LecB are altered in many of these proteins. Some of these proteins have been shown to exhibit altered sugar specificities while others are as yet uncharacterised. The existence of such homologues suggests the sugar binding specificity of the LecB protein could potentially be further diversified through mutagenesis to generate novel biomolecular recognition molecules for glycoprotein characterisation and purification applications.