The overall objective of this project is to generate novel carbohydrate binding proteins for use in glycoprotein analysis which are amenable to large scale production. The approach used here is the modification of prokaryotic glycolytic enzymes. Their enzymatic activity will be eliminated while hoping they still retain their binding capabilities. These proteins will be immobilized onto different surfaces to generate advanced bioanalytical platforms which will have huge commercial potential in the field of glycoanalysis.