Ó Fágáin, Ciarán, Cummins, Phil and O'Connor, Brendan (2011) Gel filtration chromatography. In: Walls, Dermot and Loughran, Sinéad T., (eds.) Protein Chromatography. Methods in Molecular Biology, 681 . Humana Publishers Ltd., pp. 25-35. ISBN 978-1-60761-913-0
Abstract
Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed.
Metadata
| Item Type: | Book Section |
|---|---|
| Refereed: | Yes |
| Uncontrolled Keywords: | Chromatographic methods; Microfluidics; Native protein isolation; Protein biochemistry; Protein quantitation; Purification schemes; Recombinant antibody production |
| Subjects: | Biological Sciences > Biotechnology Humanities > Biological Sciences > Biotechnology Biological Sciences > Biochemistry Humanities > Biological Sciences > Biochemistry |
| DCU Faculties and Centres: | DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology Research Institutes and Centres > Irish Separation Science Cluster (ISSC) |
| Publisher: | Humana Publishers Ltd. |
| Official URL: | http://www.springer.com/new+%26+forthcoming+titles... |
| Copyright Information: | © 2011 Springer (Humana) |
| Use License: | This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License |
| Funders: | Science Foundation Ireland, Enterprise Ireland |
| ID Code: | 17808 |
| Deposited On: | 19 Feb 2013 11:20 by Brendan O'connor . Last Modified 19 Jul 2018 14:58 |
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