Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules
Crean, Carol, Lahiff, Emer, Gilmartin, Niamh and Diamond, DermotORCID: 0000-0003-2944-4839
(2011)
Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules.
Synthetic Metals, 161
(3-4).
pp. 285-292.
ISSN 0379-6779
The attachment of antibodies onto polyaniline nanofibres using covalent chemistry was investigated for the first time. Polyaniline nanofibres were functionalised post-polymerisation to attach either amide or carboxylic acid side-groups. These templates could then be further modified to attach antibodies, specifically in this instance mouse immunoglobulin G (IgG). The resultant conjugates were characterised using a variety of techniques including infrared, UV–vis and Raman spectroscopy. Conjugates were then used to detect secondary antibodies (anti-IgG). Results from enzyme-linked immunoassay studies indi- cate successful binding of the antibody to the polyaniline nanofibres. Carboxyl functionalised polyaniline nanofibres are shown in particular to decrease non-specific binding in the immunoassay. Direct electri- cal communication between polyaniline nanofibres covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which demonstrates their potential for further development as nano-dimensional immunosensors.
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Funders:
Science Foundation Ireland under Grant No. 05/CE3/B754, EU Seventh Framework Programme for support in the form of a Marie Curie Re-Integration Grant No. PIRG02-GA-2007-224880, SFI 07/CE/I1147, Enterprise Ireland PC/2008/0149
ID Code:
17890
Deposited On:
09 Apr 2013 09:30 by
Kevin Fraser
. Last Modified 09 Jul 2019 13:40