The extensive application of monolithic columns for HPLC is severely hindered by a lack of column-to-column reproducibility. EMμ(Electroactive Monolithic μChip) is a new concept that solves the significant reproducibility problems, as well as allowing miniaturization and improving overall efficiency through electrochemically controlled dynamic separations. This novel μchip has a micro-structured monolith fabricated from intelligent, electroactive polymer. By application of a specific potential, conducting polymers such as polyaniline (PANI) can be reproducibly grown and readily fine-tuned in terms of porosity, hydrophobicity an d ionic capacity. This unique chip provides for an Electroac tive Monolithic μchip capable of multi-dimensional chromatographic separations. The monolith microstructuring (provided by templating) wi ll provide reproducibility and improve efficiency by decre asing the A-term of the Van Deemter equation. Furthermore, the use of these intelligen t materials will enable gradient control and redox reaction s to be exploited during separations of large biomolecules.