Nakatani, Yusuke, Maeki, Masatoshi 
ORCID: 0000-0001-7500-4231, Mishra, Rohit ORCID: 0000-0003-4279-4163, King, Damien, Ishida, Akihiko ORCID: 0000-0003-4100-9426, Ducrée, Jens ORCID: 0000-0002-0366-1897, Tani, Hirofumi ORCID: 0000-0002-5935-0756 and Tokeshi, Manabu ORCID: 0000-0002-4412-2144
(2015)
Liposome immunoassay based on bio luminescent detection and its application to on-chip analysis.
In: International Chemical Congress of Pacific Basin Societies (Pacifichem 2015, PAC CHEM2015), 19 Dec 2015, Honolulu, USA.
Abstract
Luc-encapsulated liposomes were employed to develop on-chip immunoassays. Straight-channel chips were better than microbead for V-cup chips to immobilize several antibodies. Straight-channel chip that has PDMS flat substrate and wider channel (1000 μm) was optimum for this assay. To detect high BL intensity, fast flow rate (300 μl/min) was better than slower flow rate because channel was filled up with substrate quickly. However, substrate volume and lysis buffer didn't affect the detecttion high BL intensity. This implies that a sufficient amount of substrate was delivered to Luc in the liposomes. The LOD of CRP in this assay was 10 ng/mL.
Metadata
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Event Type: | Conference |
| Refereed: | Yes |
| Subjects: | Biological Sciences > Biosensors Humanities > Biological Sciences > Biosensors Biological Sciences > Microfluidics Humanities > Biological Sciences > Microfluidics Engineering > Biomedical engineering Physical Sciences > Analytical chemistry Physical Sciences > Nanotechnology |
| DCU Faculties and Centres: | DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences |
| Copyright Information: | © 2015 the Authors |
| Use License: | This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License |
| ID Code: | 24246 |
| Deposited On: | 28 Feb 2020 10:11 by Rohit Mishra . Last Modified 28 Feb 2020 10:11 |
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