Nannochloropsis sp. has received increased attention by researchers in recent years due to its complexity and abundance of lipid structures. The lipids of this microalgae species have been identified to contain large quantities of neutral lipids which are capable of producing raw materials for nutraceuticals, food additives and biofuels. The production of biodiesel has received the greatest attention as there is an increase in global demand for both more fuel and more environmentally sustainable methods to produce such resources. The greatest challenges facing industries to mass produce viable products from microalgae involve the degradation of the cell wall and extracting the fatty acid of interest due to high costs. Various studies have shown that the extraction lipids from the microalgae can greatly influence the overall fatty acid composition. Different extraction methods can re- sult in recovering higher quantities of either saturated fatty acids, monounsaturated fatty acids or polyunsatu- rated fatty acids. Biodiesel production requires higher quantities of saturated fatty acids and monosaturated fatty acids as increased quantities of polyunsaturated fatty acids result in oxidation which decreases the perfor- mance of the biodiesel. Whereas, polyunsaturated fatty acids are required in order to produce pharmaceuticals and food additives such as omega 3. This review will focus on how different in-situ extraction methods for lipid and fatty acid recovery, influence the fatty acid composition of various Nannochloropsis species (oculate, gaditana, salina and oceanica). The mechanical methods (microwave, ultrasonic and supercritical‑carbon dioxide) of extraction for Nannochloropsis sp. will be critically evaluated. The use of enzymes will also be addressed, for their ability to extract fatty acids in a more environmentally friendly manner. This paper will report on the viable byproducts which can be produced using different extraction methods.