Electrochemiluminescence (ECL) based biosensors has attracted much attention since they provide high selectivity, controllability, and sensitivity. Therefore, the goal of this work was to study novel gold-based materials for the development ECL platforms using ruthenium based luminophores for future application in bacteria detection. Firstly, 3D Ti electrodes, printed using Ti alloy (Ti-6AI-4V) powder were studied and functionalized with a thin layer of gold and ECL generation was investigated with [Ru(bpy)3]2+ and the co-reactant tri-propyl amine. Results demonstrated that the presence of gold improved the diffusion on the electrode surface as well as ECL intensity, suggesting it can provide a unique and optimizable platform for their potential application for biosensors. Gold has very interesting electrochemical applications, but it also presents optical properties that can be exploited for sensing purpose, such as ECL signal amplification through optically driven plasmon excitation. With this purpose, 10 nm gold nanoparticles (AuNPs) were investigated in solution with different luminophores of [Ru(bpy)3]2+, [Ru(bpy)3-NH2]2+ and [Ru(bpy)2(phen)-NH2]2+ (separately), for the development of a SPR-ECL system. According to the fluorescence emission results, [Ru(bpy)3-NH2]2+ was chosen as the most suitable dye to develop an ECL biosensor for the DNA detection UTI causing Escherichia coli. For this, gold nanoparticles coated glassy carbon electrodes were analyse using impedance and cyclic voltammetry technique in order to investigate the suitability of the system for DNA bacterial detection. Finally, Ferromagnetic-Core/Gold-Shell NPs synthesized by thermal decomposition method were also characterised. Results confirmed that the particles were successfully synthesized and covered with gold, however, SEM images shown an aggregate size >200nm and a heterogenous shape, indicating that the synthesis protocol should be further optimized to achieve better particle properties for a magnetic ECL based biosensor. In conclusion. this thesis demonstrated the how novel platforms and luminophores could be used in conjunction with gold to develop ECL sensing systems. SPR of AuNPs coupled ECL of luminophores can be exploited to amplify the ECL signal, and overall, the analytical performance of the sensing platforms and undoubtedly, the development of highly selective and sensitive biosensors for correct detection and diagnosis of diseases such as bacterial infections.