In recent years, the real-time monitoring of primary biological aerosol particles (PBAP), like pollen and fungal spores, has gained significant attention due to their health and climactic effects. This study evaluates the efficacy of the WIBS in sampling and detecting comparisons of ambient fungal spore and pollen concentrations with the conventional Hirst volumetric method. While the total concentration of pollen and fungal spores is interesting, detecting specific allergenic pollen and fungal spore types is crucial. Results indicate that the WIBS detected and differentiated between total pollen (R2 = 0.6) and total fungal spore (R2 = 0.8) concentrations. The study also examines the correlation between ambient pollen/fungal spore concentrations and meteorological/air quality parameters. The temperature was the most influential parameter in pollen production and release, showing a strong positive correlation. Relative humidity had a strong negative correlation with pollen while showing a strong positive association with fungal spore concentrations. Wind data analysis provided geographic origin details of pollen and fungal spore concentrations, with fungal spores originating mainly from a westerly direction and pollen primarily from a North-easterly direction.