Development and characterization of nickel-NTA-polyaniline
modified electrodes
Lori, Joseph A., Morrin, AoifeORCID: 0000-0002-3031-4794, Killard, Anthony J.ORCID: 0000-0001-6953-3655 and Smyth, Malcolm R.
(2005)
Development and characterization of nickel-NTA-polyaniline
modified electrodes.
Electroanalysis, 18
(1).
pp. 77-81.
ISSN 1040-0397
The engineered addition of hexa-histidine sequences to biomolecules such as antibody fragments has been found to be
an excellent means of purifying these materials. This tagging methodology has also been extended to its use as a tool
for immobilization and orientation of antibodies on transducer surfaces. Polyvinyl sulfonate-doped polyanilne (PANI/
PVS) can be used as a mediator in amperometric biosensors. This short communication looks at the effect of nickel
chelate materials and nickel chelation on this conducting polymer and evaluates it as a potential surface for the
immobilization of his-tagged biomolecules. N-nitrilotriacetic acid (NTA) was doped into the electropolymerized
PANI/PVS at a screen-printed carbon paste electrode. The resulting NTA-PANI/PVS film was shown to have
comparable electrochemical properties of polymer without the chelating agent. When Ni 2þ was applied to the
electrode, the incorporated NTA was found to efficiently chelate the metal ions at the electrode surface.
Metadata
Item Type:
Article (Published)
Refereed:
Yes
Uncontrolled Keywords:
amperometric biosensor; protein immobilization; histidine tag; nitrilotriacetic acid; nickel; orientation;