Electrochemistry and application of a novel
monosubstituted squarate electron-transfer
mediator in a glucose oxidase-doped
poly(phenol) sensor
Iwuoha, Emmanuel I., Williams-Dottin, Avril R., Hall, Lincoln A., Morrin, AoifeORCID: 0000-0002-3031-4794, Mathebe, Gretta N., Smyth, Malcolm R. and Killard, Anthony J.ORCID: 0000-0001-6953-3655
(2004)
Electrochemistry and application of a novel
monosubstituted squarate electron-transfer
mediator in a glucose oxidase-doped
poly(phenol) sensor.
Pure Applied Chemistry, 76
(4).
pp. 789-799.
ISSN 1365-3075
Electrosynthetic poly(phenol) nanofilms were deposited in situ on platinum electrodes
in the presence and absence of glucose oxidase. The synthesis charges and currents of
the nonconducting polymer films were recorded at various applied potentials for films grown
from 25–100 mM phenol concentrations. Film parameters such as the standard rate constant
for film deposition, film thickness, and surface concentration of the poly(phenol) films were
evaluated from the cyclic and step voltammograms of the polymerization process. A novel
electron-transfer mediator consisting of monosubstituted 4-hydroxycyclobut-3-ene-1,2-dione
(squarate) was used as a mediator for Pt/poly(phenol) nano-film/GOx amperometric glucose
biosensors. Amperometric responses for 3-diphenylamino-4-hydroxycyclobut-3-ene-1,2-
dione (diphenylaminosquarate: E°′ = of +328 mV/Ag-AgCl at pH 7.0)-mediated systems
were measured by both steady-state amperometric and cyclic voltammetry. The sensor sensitivity
was calculated to be 558 nA cm
–2
(µM)
–1
.
Item Type:
Article (Published)
Refereed:
Yes
Additional Information:
Plenary lecture presented at the Inaugural Conference for the Southern and Eastern Africa Network of Analytical Chemists (SEANAC), Gaborone, Botswana, 7-10 July 2003