Protein immobilization on highly functional polymer grafted polyHIPEs
Audouin, Fabrice and Larragy, Ruth and O'Connor, Brendan and Heise, Andreas (2013) Protein immobilization on highly functional polymer grafted polyHIPEs. In: EPF 2013 European Polymer Congress, 16-21 Jun 2013, Pisa, Italy.
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PolyHIPE have proven to be useful in a large variety of applications included column filtration/separation, supported organic chemistry, as media for tissue engineering and 3D cell culture. We developed a new pHIPE platform by incorporation of a polymerizable monomer with amino group into the HIPE available for different post polymerizations. The pHIPE with amino groups on the surface (pHIPE-NH2) can be directly used for the ring opening polymerization of amino acids N-carboxyanhydrates (NCAs) to obtain pHIPE-g- polypeptides or easily converted to an atom transfer radical polymerization (ATRP) initiator for activators generated electron transfer (AGET) ATRP of tert-butyl acrylate. The polymers grafted were deprotected to form or pHIPE-g-poly(acrylic acid) with reactive groups on the surface of the pHIPE, available for bioconjugation of fluorescent proteins such as enhanced green fluorescent protein (eGFP)
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