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Development of new bioaffinity phases for glycoprotein separation and analysis

Larragy, Ruth and O'Connor, Brendan (2013) Development of new bioaffinity phases for glycoprotein separation and analysis. In: HPLC 2013, 16-20 Jun 2013, Amsterdam, Netherlands.

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Abstract

The past decade has seen an appreciation of the critical biological significance of glycosylation and the impact that glycans can have on the efficacy, stability and immunogenicity of important glycoproteins. For the biopharma industry the level of product characterization and process monitoring demanded, particularly in the context of glycosylation, is set to increase and will become an even more significant issue with the entry of biosimilar products into the biopharmaceutical arena. In order to meet these demands of the regulatory biopharmaceutical and generic producers alike require rapid , sensitive and high throughput techniques to enable detailed glycoprotein separation and analysis. It is extremely difficult to separate closely related glycoforms using standard chromatographic methods, such as ion exchange chromatography (IEX), gel exclusion chromatography (GEC) and hydrophobic interaction chromatography (HIC). Here , we report the development of a series of new bio-affinity based phases that are capable of separating closely related glycoproteins/glycoproteins. These phases get their selectivity from a number of recombinant prokaryotic bioligands called lectins. Lectins are proteins that are capable of recognizing and binding reversibly to specific glycan structures. While lectin binding affinities for monosaccharides are generally low they bind to disaccharides and more complex oligosaccharide structures with significantly higher affinities and exquisite specificity.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:Yes
Subjects:Biological Sciences > Biotechnology
Physical Sciences > Analytical chemistry
Biological Sciences > Biochemistry
Biological Sciences > Molecular biology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:17865
Deposited On:17 Jul 2013 12:07 by Brendan O'Connor. Last Modified 17 Jul 2013 12:07

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