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Analysis of water-soluble vitamins in biopharma raw materials by electrophoresis micro-chips with contactless conductivity detection

Benito-Lopez, Fernando and Vazques, Mercedes and Garcia-Cordero, Jose L. and Diamond, Dermot (2009) Analysis of water-soluble vitamins in biopharma raw materials by electrophoresis micro-chips with contactless conductivity detection. In: ICTBSB-2009 - International Conference on Trends in Bioanalytical Sciences and Biosensors, 26-28 January 2009, Dublin, Ireland.

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Abstract

Detailed information concerning the composition of the raw materials employed in the production of biologics is important for the efficient control and optimization of bioprocesses. The analytical methods used in these applications must be simple and fast as well as be easily transferable from one site to another. In that context, microchip‐based electrophoresis represents a promising tool for application in the analysis of raw materials in biologics. Using electrophoresis micro‐chips, analysis times can be reduced to seconds and high separation efficiencies can be achieved using extremely low volume samples, minimal reagent consumption and waste generation, low cost/disposability, portability and ease of mass‐production [1]. Additionally the use of Capacitively Coupled Contactless Conductivity Detection (C4D) offers a rather simple and yet sensitive method for detection of ionic species. Recently, C4D has gained much popularity as on‐chip detection in electrophoresis micro‐chips [2]. The main reason for this is that there is no physical contact of the detection electrodes with the electrolyte solution. Therefore, the integration of this detection mode within the analytical system is rather simple. Furthermore, the background noise is significantly reduced leading to lower detection limits than the conventional contact conductivity detection. Vitamins are present at very low concentrations in biopharma raw materials and are usually determined using HPLC and CE methods [3]. Electrophoresis micro‐chips are a very good alternative to these techniques due to the shorter analysis time and yet very good resolution, among others. In this paper, we present the application of electrophoresis micro‐chips with C4D detection to the analysis of water‐soluble vitamins in raw materials used for the production of biologics in bioreactors. For that purpose, hybrid PDMS/glass chips were fabricated by using standard photolithographic techniques (Figure 1). The chip structure contains an extremely long channel of 101 mm (50 x 50 μm width x depth). Figure 2 shows the setup used for vitamins detection.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:electrophoresis micro-chips;
Subjects:Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Research Initiatives and Centres > Centre for Bioanalytical Science (CBAS)
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Official URL:http://ictbsb-2009.com/
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:4072
Deposited On:15 Apr 2009 10:56 by Fernando Benito-Lopez. Last Modified 15 Apr 2009 13:55

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