Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system
Daly, Stephen J. and Dillon, Paul P. and Manning, Bernadette M. and Dunne, Lynsey and Killard, Anthony J. and O'Kennedy, Richard (2002) Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system. Food and Agricultural Immunology, 14 (4). pp. 255-274. ISSN 1465-3443
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The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible.
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