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Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system

Daly, Stephen J. and Dillon, Paul P. and Manning, Bernadette M. and Dunne, Lynsey and Killard, Anthony J. and O'Kennedy, Richard (2002) Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system. Food and Agricultural Immunology, 14 (4). pp. 255-274. ISSN 1465-3443

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Abstract

The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:aflatoxin B1; single chain Fv; phage display technology; surface plasmon resonance;
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Microbiology
Biological Sciences > Biochemistry
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Taylor & Francis
Official URL:http://dx.doi.org/10.1080/0954010021000096373
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:47
Deposited On:08 Nov 2006 by DORAS Administrator. Last Modified 08 Jun 2011 14:57

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