Production and characterization of a polyclonal antibody fornext term Os(II) and Ru(II) polypyridyl complexes
O'Connor, Máire B. and Killard, Anthony J. and O'Kennedy, Richard and Forster, Robert J. and Smyth, Malcolm R. (2006) Production and characterization of a polyclonal antibody fornext term Os(II) and Ru(II) polypyridyl complexes. Journal of Inorganic Biochemistry, 100 (7). pp. 1252-1259. ISSN 0162-0134
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The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)2dcbpy] hapten is described. Bpy is 2,2′-bipyridine and dcbpy is 2,2′-bipyridine-4,4′-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 × 108 and 5.0 × 108 M−1 for [Os(bpy)2dcbpy] and [Ru(bpy)2dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H2O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D2O/H2O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)2dcbpy] and 80% of [Ru(bpy)2dcbpy] from excited state deactivation by the aqueous solvent.
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