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Data integration for microarrays: enhanced inference for gene regulatory networks

Sîrbu, Alina, Crane, Martin orcid logoORCID: 0000-0001-7598-3126 and Ruskin, Heather J. (2015) Data integration for microarrays: enhanced inference for gene regulatory networks. Microarrays, 4 (2). pp. 255-269. ISSN 2076-3905

Abstract
Microarray technologies have been the basis of numerous important findings regarding gene expression in the last decades. Studies have generated large amounts of data describing various processes, which, due to the existence of public databases, are widely available for further analysis. Given their lower cost and higher maturity compared to newer sequencing technologies, these data continue to be produced, even though data quality has been the subject of some debate. However, given the large volume of data generated, integration can help overcome some issues related e.g. to noise or reduced time resolution, while providing additional insight on features not directly addressed by sequencing methods. Here we present an integration test case based on public Drosophila melanogaster datasets (gene expression, binding site affinities, known interactions). Using an evolutionary computation framework, we show how integration can enhance the ability to recover transcriptional gene regulatory networks from these data, as well as indicating which data types are more important for quantitative and qualitative network inference. Our results show a clear improvement in performance when multiple data sets are integrated, indicating that microarray data will remain a valuable and viable resource for some time to come.
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Data integration; Microarrays; Gene regulatory networks; Transcriptional regulation; Reverse engineering
Subjects:Biological Sciences > Bioinformatics
Computer Science > Machine learning
Mathematics > Mathematical models
Mathematics > Statistics
Physical Sciences > Statistical physics
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Engineering and Computing > School of Computing
Publisher:MDPI - Open Access Publishing
Official URL:http://www.mdpi.com/2076-3905/4/2/255
Funders:IRCSET
ID Code:20584
Deposited On:26 May 2015 10:53 by Martin Crane . Last Modified 19 Nov 2021 11:42
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