Analysis of sweat offers a wealth of information related to hydration, nutrition, and athletic performance. The conductivity of sweat can be directly related to sodium chloride concentration, as these are the most abundant electrolyte ions present in sweat [1]. Individuals affected by cystic fibrosis contain a higher concentration of Cl- ions in their sweat. Prescription medications for the disease reduce Cl- concentrations in sweat, and as a result, the efficacy of these medications can be monitored non-invasively with sweat collection. In previous work, we have demonstrated the use of capacitively coupled contactless conductivity detection (C4D) for testing the response of commercial gold microelectrodes to NaCl solutions using multiple sampling platforms [2]. This work presents the optimization of channel and sampling volumes to calculate and minimize the sensor’s response time for applications in wearable sweat sensing. In preparation for on-body testing, the functionality of the chip was optimized for relevant flow rates of sweat. Sweat rate can vary drastically depending upon the subject and body part. Additionally, those affected by cystic fibrosis have difficulty exercising for extended periods of time. Due to these restrictions, the volume of sweat needed to produce a signal is of critical importance. In this work, PDMS microchannels were created which minimized platform volume for on-body analysis. Using varying concentrations of NaCl solutions (10 mM - 130 mM) and the average flow rates for the arm (730 g/m2h), back (797 g/m2h) and forehead (894 g/m2h), a calibration curve was created and the average response time was calculated for each body location. Finally, tests were completed with artificial sweat and compared to the calibration curves. [1] Lezana, J. L.; Vargas, M. H.; Karam-Bechara, J.; Aldana, R. S. and Furuya, E. Y. J. Cyst. Fibros., 2003, (pp. 1-7). [2] Deignan, J.; Florea, L; Coyle, S; and Diamond, D. MicroTAS 2014, 2014 (pp. 2184-2186). Science foundation Ireland under the Insight initiative, grant SFI/12/RC/2289